Commensal Escherichia coli isolates are phylogenetically distributed among geographically distinct human populations

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Commensal Escherichia coli isolates are phylogenetically distributed among geographically distinct human populations.

An intraspecies phylogenetic grouping of 168 human commensal Escherichia coli strains isolated from the stools of three geographically distinct human populations (France, Croatia, Mali) was generated by triplex PCR. The distributions of seven known extraintestinal virulence determinants (ibeA, pap, sfa/foc, afa, hly, cnf1, aer) were also determined by PCR. The data from the three populations we...

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Autotransporter-encoding sequences are phylogenetically distributed among Escherichia coli clinical isolates and reference strains.

Autotransporters are secreted bacterial proteins exhibiting diverse virulence functions. Various autotransporters have been identified among Escherichia coli associated with intestinal or extraintestinal infections; however, the specific distribution of autotransporter sequences among a diversity of E. coli strains has not been investigated. We have validated the use of a multiplex PCR assay to...

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Large-scale population structure of human commensal Escherichia coli isolates.

The study of several Escherichia coli intestinal commensal isolates per individual in 265 healthy human subjects belonging to seven populations distributed worldwide showed that the E. coli population is highly structured, with major differences between the tropical and temperate populations.

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Genetic variation among Escherichia coli isolates from human and calves by using RAPD PCR

Background: Various strains of Escherichia coli (E. coli) are known as major causes of intestinal and extraintestinal infections in humans and various animal species. Molecular methods are important for the identification of bacterial isolates and nucleotide sequence variations, as well as information on tracking bacterial agents related to the outbreaks, the frequency of the bacterial genetic ...

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Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates.

Despite the critical role of plasmids in horizontal gene transfer, few studies have characterized plasmid relatedness among different bacterial populations. Recently, a multiplex PCR replicon typing protocol was developed for classification of plasmids occurring in members of the Enterobacteriaceae. Here, a simplified version of this replicon typing procedure which requires only three multiplex...

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ژورنال

عنوان ژورنال: Microbiology

سال: 2001

ISSN: 1350-0872,1465-2080

DOI: 10.1099/00221287-147-6-1671